Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 22)

Table 4. Recommended application buffer
for specific samples.
Target Bio-Scale Mini
Sample Compound Cartridge Buffer
Rabbit, rat, goat, IgG DEAE Blue A
or sheep serum
Human serum IgG DEAE Blue B
Serum Albumin Blue C
Physiological fluids Enzymes Blue Varies
4.1 Sample Preparation
Proper pH and ionic strength are necessary for
consistent and reproducible results. Sample can be
exchanged into the starting buffer or diluted to the
starting buffer's concentration. This can be
achieved by diluting the sample to the ionic strength
of the starting buffer, dialyzing against the starting
buffer, or exchanging it into the starting buffer.
Buffer exchange can be accomplished using a
Bio-Scale Mini P-6 cartridge, Bio-Spin
® 6 or Bio-Spin
30 column, Econo-Pac ® 10DG desalting column, or
Bio-Gel ® P-6 DG gel filtration gel (seeTable 5). The
choice of product will depend on sample volume. All
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