Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 25)

Section 5
Specific Purification
Protocols
Several specific application protocols have been
developed using dye affinity supports. Examples
follow.
5.1 Purification of IgG from Serum or
Ascites With the Bio-Scale Mini DEAE
Affi-Gel Blue Cartridge
IgG can be isolated from serum or ascite samples
using the Bio-Scale Mini DEAE Affi-Gel Blue
cartridge. The resulting purified IgG fraction may
contain a residual amount of transferrin.
1. Equilibrate the cartridge in application buffer (A
or B).
2. Apply the prepared sample to the cartridge.
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