Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 24)

4.2 General Purification Protocol
Affinity chromatography can be performed using
isocratic elution or by increasing salt or pH gradients
to fractionate the sample components. For best
results, and increased cartridge life, samples, and
buffers should be degassed and filtered through a
0.45 µm filter. Table 4 references selected buffers
for some specific applications. Buffer formulations
are listed in Table 3.
4.3 Scaling Up the Separation
For quick scale-up, two or three cartridges of the
same type can be connected in series.
Backpressure will increase with cartridges in series,
so care should be taken to maintain pressures
≤ 10 psi. Bio-Scale Mini Affi-Gel Blue and DEAE
Affi-Gel Blue cartridges are available in 5 ml format.
The Affi-Gel Blue and DEAE Affi-Gel Blue media are
also available in 100 ml bottles for scaling up
methods developed using the cartridges. In addition,
Bio-Rad carries an extensive line of empty
chromatography columns from laboratory scale to
process scale.
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