Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 29)

proceed to step 5. If the enzyme is not bound,
alter the application conditions: change the pH,
change the buffer, or decrease ionic strength.
5. The enzyme can be eluted with a salt gradient
2 or with a competitive eluant such as a
cofactor. Examples of salt gradients include
0.05 to 1.5 M NaCl
3, 4, 5 or 0.0 to 3.0 M KCl. 6,
7, 8 Request technical bulletin 1107 for more
information on elution buffers for enzyme
purification.
6. Regenerate the cartridge as recommended in
Section 6.1, Cleaning the Cartridge.
Section 6
Care of the Cartridge
6.1 Cleaning the Cartridge
After each use, both Bio-Scale Mini dye affinity cartridges
require thorough cleaning and regeneration to
remove bound contaminants. Referring to Table 4
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