Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 21)

Table 3. Buffer formulations.
Application Buffers
A. 0.028 M NaCl, 0.020 M Tris-HCl, pH 8.0
B. 0.020 M K
2
HPO
4
, pH 8.0
C. 0.020 Na
2
HPO
4
, pH 7.1
Elution Buffers
D. 0.4 M K
2
HPO
4
, pH 8.0
E. 1.4 M NaCl, 0.020 M Tris-HCl, pH 8.0
F. 1.4 M NaCl, 0.020 M Na
2
HPO
4
, pH 7.1
Regeneration Buffers
G. 1.4 M NaCl, 0.10 M acetic acid, pH 3.0, 40% v/v isopropyl
alcohol
H. 1.5 M sodium thiocyanate in application buffer A, B, or C
I. 2.0 M guanidine-HCl in application buffer A, B, or C
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