Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 30)

for buffers, bound contaminants may be removed
by following the procedure below:
1. Set the pump flow rate to 2.0 ml/min.
2. Wash the cartridge with 10 ml of elution buffer
(E or F).
3 Wash with 10 ml regeneration buffer (H or I).
4. Wash with 20 ml application buffer (A, B, or C).
5. Reduce the flow to 1.0 ml/min.
6. Continue with sample application.
6.2 Storage
Bio-Scale Mini dye affinity cartridges should be
stored at 4 °C in 0.020 M sodium phosphate buffer
containing 0.05% sodium azide. Perform steps 1–3
in the Cleaning the Cartridge section, then wash
with storage buffer.
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