Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 27)

flow rate and gradient profile. Request bulletin 1092
for further information on the purification of serum
proteins using DEAE Affi-Gel blue gel.
5.3 Removal of Albumin from Serum With
the Bio-Scale Mini Affi-Gel Blue Cartridge
The Bio-Scale Mini Affi-Gel Blue cartridge can
provide a simple first step in the purification of many
serum proteins by removing the major serum
component, albumin.
1. Equilibrate the cartridge in application buffer (C).
2. Apply the prepared sample to the cartridge.
3. Wash the cartridge with 10–15 ml of application
buffer (C). The effluent from this step contains
the serum proteins minus most of the albumin.
4. Optional: Most of the bound albumin can be
eluted by washing the cartridge with 10-20 ml
of elution buffer (F).
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