Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges - Manual (Page 17)

Section 4
Preparing a Cartridge
For Use
Bio-Scale Mini Macro-Prep High Q, Macro-Prep
High S, and Macro-Prep DEAE cartridges contain
20% ethanol (v/v) as the storage solution. The fully
hydrated support is ready to use after equilibrating
the cartridge in the buffer of choice. To perform
buffer exchange, connect the cartridge to a liquid
chromatography system or peristaltic pump and
condition it as instructed below:
1. Set pump flow rate to 3.0 ml/min (731 cm/hr)
for the 1 ml cartridge or 6.0 ml/min (288 cm/hr)
for the 5 ml cartridge.
2. Wash the cartridge with degassed low-salt
buffer for 2 min.
3. Wash the cartridge with degassed high-salt
buffer for 5 min.
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