Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges - Manual (Page 22)

Section 5
Care of the Cartridge
5.1 Regeneration
After each use, the cartridge should be regenerated
with the appropriate salt, in most cases 1–2 M NaCl
in the presence of buffer. Wash with 2 to 4 column
volumes of the buffered high salt solution. This
reduces the potential for protein precipitation when
selecting acid as a cleaning agent.
5.2 Cleaning
After repeated use, an ion exchange cartridge may
require thorough cleaning and regeneration to
remove bound contaminants. Acceptable cleaning-
in-place (CIP) reagents include 1% acetic acid/1%
phosphoric acid with 0.4 M NaCl, up to 30% acetic
acid, 1% Triton X-100, up to 70% ethanol or 30%
isopropyl alcohol, 8 M urea, and 6 M guanidine-HCl.
Any of these agents can be combined in an
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