Table 3. products for buffer exchange – Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges User Manual
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Table 3. Products for buffer exchange.
Sample
Recommended
Volume
Product
Use
Catalog #
50–100 µl
Bio-Spin 6 column
Desalting proteins
732-6002
≥ 6 kD
50–100 µl
Bio-Spin 30 column
Desalting proteins
732-6006
≥ 30 kD
100 µl–3 ml
Bio-Scale Mini P-6
Desalting proteins
732-4502
cartridge
≥ 6 kD
Up to 3 ml
Econo-Pac 10DG
Desalting proteins
732-2010
desalting column
≥ 6 kD
Unlimited
Bio-Gel P-6DG gel
Desalting proteins
150-0738
≥ 6 kD
4.2 General Purification Protocol
Ion exchange chromatography is usually performed
using increasing salt gradients or pH gradients to
elute the sample components. For best results and
to increase cartridge life, samples and buffers should
be degassed and filtered through a 0.45 µm filter.
Common buffers for cation and anion exchange
chromatography are listed in Table 4. An appropriate
starting point for purifying samples is a linear
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