Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges User Manual
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Table 2. Macro-Prep High Q & S and
Macro-Prep DEAE specifications.
Properties
High Q
High S
DEAE
Type of ion exchanger
Strong anion
Strong cation
Weak anion
Functional group
-N+(CH3)3
-SO3-
-N+(C2H5)2
Total ionic capacity
400 ± 75 µeq/ml
160 ± 40 µeq/ml 175 ± 75 µeq/ml
Dynamic binding capacity*
≥37 mg BSA/ml
≥49 mg IgG/ml ≥30 mg BSA/ml
Shipping counterion
Cl-
Na+
Cl-
Chemical stability
1% SDS, 24 hr
Yes
Yes
Yes
6M guanidine-HCl, 24 hr
Yes
Yes
Yes
pH stability
1–10
1–12
1–10
Total organic carbon in ppm, after 24 hours at 22°C
0.1 M HCl
0
4
0
0.2 M H
3
PO
4
0
13
0
0.01 M NaOH
28
N/A
N/A
0.1 M NaOH
200
31
26
1.0 M NaOH
337
49
121
Antimicrobial agent
20% ethanol
20% ethanol
20% ethanol
Regeneration
1–2 M NaCl
1–2 M NaCl
1–2 M NaCl
Storage conditions
20% ethanol
20% ethanol
20% ethanol
* 10% breakthrough capacity determined in a 1.1 x 20 cm column.
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