Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges - Manual (Page 21)

Table 4. Common buffers for ion exchange
chromatography.
Type of Buffering Buffer Range
Cation Exchanger
Acetic acid 4.8–5.2
Citric acid 4.2–5.2
HEPES 7.6–8.2
Lactic acid 3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
Pivalic acid 4.7–5.4
TES 7.2–7.8
Tricine 7.8–8.9
Anion Exchanger
Bicine 7.6–9.0
Bis-Tris 5.8–7.2
Diethanolamine 8.4–8.8
Diethylamine 9.5–11.5
L-Histidine 5.5–6.0
Imidazole 6.6–7.1
Pyridine 4.9–5.6
Tricine 7.8–8.9
Triethanolamine 7.3–8.0
Tris 7.5–8.0
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