Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 10)

2.3 System Check Maintenance Procedures
2.3.1 Maintenance Procedure 1 –– High Voltage (HV) Conditioning
This procedure helps to decontaminate the surfaces of the ProteinChip
instrument and is essential for obtaining correct test results. Run this
procedure weekly using any blank ProteinChip array (or an array that
has already been used). The procedure takes 1–2 hr to complete.
Therefore, to save time, we recommend running this protocol at the end
of the day prior to running the remaining tests. There is no need to stay
with the instrument while the protocol is running.
1. Launch ProteinChip data manager software, select the instrument,
and click Start.
2. Insert a blank ProteinChip array into the instrument. If blank arrays
are not available, use an expired or used ProteinChip array.
Note: If using an Enterprise Edition instrument, place a cassette
filled with used ProteinChip arrays into the instrument. Blank arrays
maybe used as long as at least one array in the cassette has a bar
code.
3. Open the Protocol Mode tab and select Instrument > Quick Run.
4. The Quick Run Protocol(s) dialog opens. Select the SoftHV
protocol you saved during file and protocol setup, step 3 and click
Run.
5. After completion of the protocol, an “Acquisition Error” message
appears. This is expected. Click OK.
6. Record the operator name and date in the ProteinChip SELDI system
check form.
2.3.2 Maintenance Procedure 2 –– Detector Calibration
This procedure uses the ProteinChip detector calibration array to adjust
the detector voltage. These adjustments are based on a rolling average
that stabilizes the gain, improving spectral reproducibility over the lifetime
of the detector. This procedure is essential for obtaining correct test
results and, when run on a weekly basis, is designed to improve
reproducibility as the detector ages.
Each run uses a single spot on the array, and each spot can only be
used once. Store the array in its original packaging and in a dry, dark
location. Record usage data directly on the packaging.
The procedure requires 45 min to 4 hr to complete, depending on the
state of the instrument.
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