Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 9)

Step 3
Set Up the
High Voltage (HV)
Conditioning Routine
6
1. Insert a blank ProteinChip array into the reader. If blank arrays are not
available, use expired arrays or arrays that have already been used.
a. For the Enterprise Edition instrument: place a cassette filled with
12 blank or used arrays into the instrument.
b. For the Personal Edition instrument: insert a blank ProteinChip
array.
2. In ProteinChip data manager software, select the instrument and click Start.
3. In the Protocol Mode tab, select Instrument > Quick Run.
4. Select all eight spots, then set up a Quick Run protocol as shown below:
a. For the Enterprise Edition instrument:
RC3 HV pos 15 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 20 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 30 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV neg 15 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV neg 20 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV neg 25 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV neg 30 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 15 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 20 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 30 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots
b. For the Personal Edition instrument:
RC3 HV pos 15 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 20 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 30 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 15 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 20 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 30 kV conditioning, partition 1 of 8, all 8 spots
RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots
5. Click Save in the Quick Run dialog, and save the protocol as SoftHV in the
Quick Run directory (C:\\XXXXXXX). The Quick Run directory location is
defined by the user. The default location, "My Documents", will work as
well as any other location that is easy to remember.
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