Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 7)

Chapter 2
Use of the ProteinChip System Check Kit
2.1 Overview
This chapter outlines the protocols used to perform the procedures and
tests that comprise the ProteinChip system check kit. Please note the
following:
• Export data acquired during testing to the ProteinChip SELDI system
check form using the export instructions described in Appendix A
• Protocol transfer from the CD to ProteinChip data manager software
needs only to occur once; however, new files must be created each
time a test is run
• Neither spreadsheets nor protocols are write-protected. Overwriting
may result in erroneous results and calculations
• Always perform the two maintenance procedures before running any
of the tests
• Maintenance procedure 2 (detector calibration) is designed to
standardize and stabilize detector performance over time by resetting
any drift in the response due to natural component aging. First or
delayed use of this procedure may alter system response after
calibration. Do not run this procedure within a series of related
experiments if it has been more than two weeks since the last
detector calibration
• All tests require peak measurements that are compared to specification.
Incorrect peak selection may lead to erroneous results. While
selecting peaks for measurement, zoom in on them so that they
resemble the peaks in the examples provided here
• When running single ProteinChip arrays on the ProteinChip instrument,
Enterprise Edition, always fill empty locations in the cassette with
blank, expired, or used arrays. Blank arrays are available as
components of the ProteinChip cassette-compatible bioprocessor
(catalog #C50-30011)
• The ProteinChip detector calibration array and ProteinChip peptide
standard array can be used multiple times. After opening, store
these arrays in a dry and dark place and in their original packaging
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