Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 8)

• When running protocols, you specify how many sections (partitions)
the spot is divided into for sampling. You also specify which of these
partitions should be used. For spectra intended for analysis, no more
than four ( "of 4") partitions should be used, with 10 shots/pixel
2.2 File and Protocol Setup
Complete this procedure the first time you use the ProteinChip system
check kit. This procedure sets up the file structure necessary for storing
the protocols and creates the routines for high voltage conditioning
(maintenance procedure 1).
Task
Step 1
Import Protocols
From CD
Step 2
Create the File
Structure
Note: Create new folders each
time the tests are run. The
instructions in this manual
assume you are following the
convention described here.
However, any file structure and
naming convention may be
used.
5
Import the protocols supplied on the CD into the
Protocols folder of ProteinChip data manager
software:
1. Insert the CD into the computer and launch
ProteinChip data manager software.
2. Select Protocols > Protocols > Import
Protocols.
3. Use the Import Protocols dialog to import
all protocols (files with a .ptx extension) from
the CD to the Protocols folder.
Note: The protocols are NOT write-protected. Do
not alter them.
Create a new folder for each test (1–2) to be run:
1. In the Explorer pane of ProteinChip data
manager software, open the Projects folder
and select File > New > Folder.
2. Create a folder, system check_####, where
#### is the serial number of the instrument.
3. Click on this folder and select File > New >
Folder. Create a separate folder for the date
of the test.
4. Click on the folder created in step 3 and
select File > New > Folder. Create a
separate folder for each of the following
tests:
• Test 1 Detector sensitivity
• Test 2 Mass drift and resolution 5.96 kD
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