Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 5)

Table 1. Summary of procedures and tests.
C Co on nssu um maab bllee E Essttiim maatteed d T Tiim mee
T Tiittllee U Usseed d R Reeq qu uiirreed d D Deessccrriip pttiio on n
IIn niittiiaall S Seettu up p File and protocol 20 min Complete this procedure
setup the first time you use the
ProteinChip system check
kit. This procedure creates
the file structure necessary
for storing the protocols
and creates the routines for
high voltage conditioning.
M Maaiin ntteen naan nccee High voltage N/A 1–2 hr HV conditioning helps to
P Prro occeed du urree 1 1 (HV) depending on decontaminate the surfaces
conditioning instrument in the instrument.
M Maaiin ntteen naan nccee Detector ProteinChip 45 min to 4 hr This procedure uses the
P Prro occeed du urree 2 2 calibration detector depending on ProteinChip detector
calibration instrument calibration array to adjust
array the detector voltage. These
adjustments are based on a
rolling average that stabilizes
the gain, improving spectral
reproducibility over the
lifetime of the detector.
T Teesstt 1 1 Detector ProteinChip 30 min to 1 hr This test uses the
sensitivity detector ProteinChip detector
qualification calibration array to
array measure the
signal-to-noise ratio
(S/N) of immunoglobubulin
(IgG) at two different
concentrations (10 fmol
and 140 fmol).
Measurements are
compared to a specification,
and a pass/fail disposition is
obtained.
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