Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 16)

2.4.2 Test 2 — Mass Drift and Resolution 5.96 kD
This test uses the ProteinChip peptide standard array to measure the
mass drift and resolution of insulin. These measurements are compared
to a specification, and a pass/fail disposition is obtained.
Array Type ProteinChip peptide standard array
Usage Information Each test uses 1 partition on all 8 spots
on the array. The array can be used up to
20 times (5 times on each of 4 partitions).
Store the array in the original packaging
and record usage information in the
usage chart provided and on the package.
Laser optimization steps use 1 of 15
partitions, and these reads do not need
to be recorded or monitored.
Expected Testing Time 20 min to 1 hr
Pass/Fail Specification Mass drift: pass if less than 7 Da
Resolution: pass if average is greater
than 750
1. Insert the ProteinChip peptide standard array into the instrument. If
you are using an Enterprise Edition instrument, place the array into
a cassette, fill the remaining slots in the cassette with blank or used
arrays, and place the cassette into the instrument.
2. In ProteinChip data manager software, click Start and open the
Protocol Mode tab.
3. Select Instrument > Preferences. In the Preferences dialog, click
“…”. The Select Folder dialog opens.
4. Select Projects > system check_####/ Date/Test 2 Mass Drift and
Resolution 5.96 kD, where #### is the serial number of the
instrument.
5. Optimize the laser intensity. Run protocol “Test 2 Mass drift and
resolution 5.96 kD” on any partition (“n”) of 15 on spot D (for example,
1 of 15, 2 of 15, 3 of 15, etc). Adjust the laser energy until the
height of the insulin peak at 5,963 Da is 100–200 µA. This
optimization step does not need to be repeated on subsequent runs
of test 2 unless the peak intensity falls too low.
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