Shellab BACTRON600 - Manual (Page 43)

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OPERATION (CONTINUED)
Manual Cycle
The manual cycle is intended as a backup for use in the event the auto cycle is not functioning.
1. Verify that the inner pass box door is closed and secured.
2. Open the outer pass box door. The Pass Box Aerobic Light will illuminate, indicating that
the pass box chamber has been exposed to the aerobic atmosphere of the room.
3. Load the pass box slider shelf with items to be introduced into the workspace chamber.
4. Close and secure the outer door. The Pass Box Doors light will illuminate when both doors
are closed. Do not manually cycle the pass box if the Pass Box Doors light is not
illuminated!
Note: Contact Sheldon Technical support if the Air Lock Doors light will not illuminate when both
doors are closed and secured.
5. Depress the Manual Gas – OFF – VAC switch to the right (VAC). Hold the switch down
until the vacuum gauge display shows -17 inHG to -20 inHg of air pressure in the pass box.
Note: If the Chamber Gas light illuminates frequently and disrupts operation of the vacuum pump
while vacuuming down the chamber, release the Manual Gas – Off – Vac switch. Immediately
cease vacuuming if the water in the manometer gauge begins to bubbles. In both cases there
may be a leak along the inner pass box door. Continuing to vacuum may seriously damage
the BACTRON. Contact Sheldon Technical Support for assistance.
6. Depress the Manual Gas – OFF – VAC switch to the left (GAS) to inject AMG into the pass
box. Hold the switch until the vacuum gauge display reads approximately -4 inHg. This
completes one sub-cycle. Note that manual injections only draw from the GAS 1 IN port.
7. Repeat step 5 twice more. On the third and final sub-cycle, hold the Manual – Gas – Off
switch to the left until the vacuum gauge display reads 0. The pass box has now been
restored to the room’s atmospheric pressure, and the pass box interior door can now be
opened.
8. The Anaerobic indicator light will not illuminate following the completion of a manual cycle.
It only illuminates after the auto cycle completes.
9. Do not open the outer pass box door while the inner pass box door is opened!
10. After removing items from the slider shelf, return it to the pass box chamber. Close the
inner pass box door. This safeguards against inadvertently introducing oxygen into the
workspace chamber if someone opens the outer door later on.
Note: Failure to cycle the pass box to the standards described above risks allowing significant
amount of aerobic atmosphere into the workspace chamber.