Shellab BACTRON600 - Manual (Page 44)

44 | P a g e
OPERATION (CONTINUED)
L
OADING THE
W
ORKSTATION
Sheldon Manufacturing recommends waiting 24 hours after establishing an anaerobic atmosphere
before loading samples into the unit.
Airtight containers can introduce significant amounts of oxygen into the anaerobic environment of
the BACTRON. Whenever possible, closed containers placed in the pass box should be loose-
capped or ventilated to allow the pass box cycles to draw oxygen from the containers. Syringes
should be loosened and sealed containers partly opened, if permitted by your laboratory or
production protocol.
The pass box sliding shelf can hold and transport up to 252 plates. Place samples and other media
containers on the shelves inside each incubator as evenly spaced as possible. Good spacing
allows for atmosphere circulation and a higher degree of temperature uniformity. If the anaerobes
sensitive to heat are being cultivated, it may be necessary to place an empty petri dish or plate at
the bottom of each stack.
This concludes the Setting up the BACTRON portion of the Operation Section.