Shellab BACTRON600 - Manual (Page 46)

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OPERATION (CONTINUED)
C ALIBRATE THE I NCUBATOR T EMPERATURE DISPLAY
Temperature calibrations are performed periodically to ensure that the control panel temperature
display matches the actual temperature inside a BACTRON incubator. Each BACTRON incubator
is calibrated at the factory to 37°C. Calibrate as often as required by your laboratory or production
protocols, or regulatory requirements.
Allow the incubator at least 24 hours to stabilize heated at its operational set point, with the
incubator doors closed, before performing a calibration. Always use a certified temperature sensing
reference device that is regularly calibrated to 0.1°C by an independent party to conduct
temperature verifications or calibrations. For best results use a device with a thermocouple or other
temperature probe.
1. Place the temperature sensor of a calibrated reference device inside the incubator as close
as possible to the chamber’s geometric center. Check that the sensor is not in direct
contact with the shelving.
Note: A thermocouple sensor probe’s sleeve may be taped to the shelving, as long as the exposed
copper end is 2 inches (5cm) above the shelf. An exposed sensor probe in direct contact
with the shelving may experience heat sinking, which can result in an inaccurate temperature
reading.
2. Allow the temperature to re-stabilize after closing the door.
a. The reference device ’s temperature measurement should not change for at
least one (1) hour after a significant disruption for the chamber to be
considered stabilized.
3. If the reference device’s and the incubator’s temperature readings are the same, or the
difference between the two falls within the acceptable range of your laboratory protocol
after one (1) hour of stability, the incubator is calibrated.
4. If there is a difference between the two (2) readings, and that offset falls outside your
laboratory protocol ’s acceptable range , adjust the incubator’s temperature display to match
the reference device’s reading. See the next step.
a. If the door was briefly opened for a few seconds to take a reading, wait fifteen
minutes for the temperature to stabilize before correcting for the offset. If the
opening was longer, wait one (1) hour for the temperature to stabilize.
5. Press and hold both the UP and DOWN arrows simultaneously. The Temperature Display
of most models will flash the letters “CO” when the contro ller enters calibration mode.
6. The display will continue flashing and display the current temperature value. Adjust the
current value until it matches the reference device’s temperature reading.
7. After matching the unit’s current value to that of the reference device, wait five seconds.
The incubator’s Temperature Display will cease flashing, and the incubator will begin
adjusting to compensate for the entered temperature offset.
Procedure continued on next page