Specifying wash cycle operations – Dynex OPSYS MW 91000051 User Manual

Page 53

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Creating a New Wash Protocol

Specifying Wash Cycle Operations

The final step in creating a wash protocol is to specify individual operations
within the wash protocol.

An example of a wash protocol is shown in 45. In this example, the dispense
wash pins are purged, and then a Dispense and Aspirate are carried out
twice on each of the 12 strips (columns) of a 96-well Microtiter

®

plate.

Finally, after all 12 strips have been washed twice, the contents of the
Microtiter plate are allowed to soak (equilibrate) for another 600 seconds.

PURGE

Purge the dispense pins.

↓

MOVE

(2 Cycles)

Specify the starting strip and ending strip for each cycle
and the number of cycles to be performed.

↓

DISPENSE

(3 Times)

For each strip, perform dispense three times.

↓

ASPIRATE

For each strip, perform one aspiration cycle.

↓

END LOOP

End the cycle.

↓

SOAK

After the two Move cycles have been completed, allow
the contents to equilibrate for 600 seconds.

↓

END

End the wash protocol.

Figure 9. Example of a Wash Protocol

The procedures for specifying the steps in this and other wash protocols are
described on the following pages.

Opsys MW™ User’s Guide

45

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