Hudson Robotics SOLO User Manual

Page 32

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The file for a 384-well plate would have 16 rows of 24 values each.

For multi-tip operation only values for wells addressed by first tip of

multi-tip head are taking in consideration. Tips on the head are

counted from left to right. See bellow file list for aspirating with 12

tip head (‘P12’) from 96-well plate:

50, 50, 50, 50, 50, 50, 50, 50, 50, 50, 50, 50

42, 42, 42, 42, 42, 42, 42, 42, 42, 42, 42, 42

0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0

0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0

0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0

0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0

20, 20, 20, 20, 20, 20, 20, 20, 20, 20, 20, 20

10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10

Well positions that have zero (or negative) volume values will simply

be skipped. The SOLO will aspirate from the number of non-zero

wells determined by the “Multiple Wells” setting, then, if inside a

“Loop” , will start at the well following the last one aspirated during

the previous execution of this step. The volumes to be aspirated will

appear in their respective well locations in the plate image across the

bottom of the display.

Aspirate from ‘Named’ Point:

The volume to be aspirated from a point, such as a vial location, can

also be imported from a comma-separated text file, but for this type of

position, the values can be in any number of rows/columns, and are

read in left-to-right, top-to-bottom order. As in the case of

aspirating from a plate position above, any zero (or negative) volume

values will simply be skipped. The SOLO will aspirate the next non-

zero volume in the file. If inside a “Loop”, it will use the value

following the last one aspirated during the previous execution of this

step. A complete list of the values to be aspirated can be viewed in the

dropdown’ list inside the “Aspirate Volume(uL)” box.

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