ChemoMetec NC-3000 - Manual (Page 31)

991-3003 Rev. 1.0 August 2013 31
Image Overlay
An image overlay may be enabled with the button near the right bottom corner of the image. It
performs two kinds of cell marking on the displayed image:
 When an analysis is completed successfully, cells that are included in the analysis are marked with a
square.
 When a large scatter plot or a large histogram is open in the Plot Manager the cells inside or
outside the selected marker or gate respectively, can be marked with the overlay squares by
selecting ‘Add Cells Inside Selected Gate to Image Overlay’ or ‘Add Cells Outside Selected Gate to
Image Overlay’.
The cells are marked with squares and this overlay can be toggled on and off by clicking the button.
When one of the two overlay functions has been used it is possible to choose cells in the Main Window by
holding the Ctrl button down and clicking on individual cells in the image. This will mark the last selected
cell with a yellow square and previously selected cells with a pink square. Selected cells will be marked in
Large Scatter Plots .
The selected cells for the image overlay can be unselected by double clicking in the image, by right clicking
the button or the large plot and selecting ‘Delete Image Overlay’ or by right clicking on a large plot and
selecting ‘Delete Image Overlay’.
See the
Large Scatter Plot for details about polygons and cell gating. See the Large Histogram Plot for
details about markers and cell gating.