ChemoMetec NC-3000 User Manual

Page 38

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991-3003 Rev. 1.0

August 2013

38

Row number: The row number is shown in the upper left corner.

Data source indicator: The data source of the cell population in this row is shown with a small colored
square to the left of the ‘save’ button:

1. Grey: Indicates that the cell population has been loaded from data embedded in the image file, i.e.

data which was obtained when the image was originally acquired and analyzed by running a
protocol.

2. Green: Indicates that the data has been loaded from the main window, and that the main window

still holds the image file in memory. This is referred to as Main data (synchronized). This population
is loaded instead of embedded data if the main window holds cell population data for that
particular image file at the time when the row is added to the Plot Manager.

3. Orange: Same as for green, but the main window no longer holds the same image file in memory.

This is referred to as Main data (not synchronized).

The ‘save’ button saves the post processing results in a *.cmpp file which is automatically given the
same name and same location as the *.cm image file.

Note: Any already existing post processing result for this CM-file will be overwritten.
For all analytical assays a *.cmpp files is saved automatically when running the analysis, hence it is
not possible to do further post processing or saving of data in 21CFR Part 11 mode since this will
overwrite existing data.
To get saved *.cmpp files with data analysis in 21 CFRP part 11 mode, it is advised to use adapted
protocol containing a master template for the analysis of data.


File name: The name of the image file is shown with vertical font in the left side of the row window.

Check box: Check the check box under the ‘save’ button if you want the row to be a target for the apply
master function.

Click the ‘add histogram’ button to add a new

Histogram Plot

to the end of this row.

Click the ‘add scatter plot’ button to add a new

Scatter Plot

to the end of this row.

Click on this button to open the

Compensation Window

.


Right-click options: Right click on the row window to see the options:

Show Image: Loads the rows corresponding image file (cm file) into the

Main Window

.

Save As Master: Saves this rows post processing as a Master for post processing. The saved master
can be loaded into subsequent Plot Manager sessions where the post processing can be replicated
to other rows.

Reload Post Processing: Reloads the last saved post processing of the file.

Move Row Up: Swaps row position and number with the previous visible row.

Move Row Down: Swaps row position and number with the next visible row.

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