ChemoMetec NC-3000 - Manual (Page 41)

991-3003 Rev. 1.0 August 2013 41
6. Cancel: Closes the large plot without saving the changes.
OK: Closes the large plot and saves the changes.
7. Line type and colors: These may be set with the controls in the upper right corner of the plot
window after an additional histogram has been overlaid to an existing histogram.
8. Data source indicator: A small colored square in the upper left corner of the plot area indicates the
source of the cell population (see further details in the section Row Window in Plot Manager).
Right-click options: Right-click inside the plot area when no markers are selected to get a context menu
offering the following options:
 Paste Marker: Inserts a copied marker into the histogram plot.
 Show Gate Counts: Toggles between displaying counts and percents for the gates.
 Copy Histogram: Copies the histogram curve.
 Paste Histogram: Inserts a copied histogram curve into the histogram plot.
Delete Image Overlay: Unselects cells added to the Image Overlay .
Depending on the state of the large scatter plot, some of the above options may be dimmed or be
replaced with an alternative option.
Right-click options: Right-click inside the plot area when a marker is selected to get a context menu offering
the following options:
 Paste Marker: Inserts a copied marker into the histogram plot.
 Copy Selected Gate: Copies the selected gate.
 Copy Histogram: Copies the histogram curve.
 Paste Histogram: Inserts a copied histogram curve into the histogram plot.
 Add cells inside marker to image overlay: Selecting this option will add cells inside the selected
marker to the image overlay, causing these cells to be marked with a non-filled, enclosing
square.
 Add cells outside marker to image overlay: Selecting this option will add cells outside the
selected marker to the image overlay, causing these cells to be marked with a non-filled,
enclosing square.
 Delete Image Overlay: Unselects cells added to the Image Overlay .
Depending on the state of the large histogram plot, some of the above options may be dimmed or not
present.
Note: Adding cells to the image overlay is cumulative when it is done from the same plot. When adding
cells to the image overlay from another plot, the overlay is first cleared. Consequently if you use this to
select both inside and outside cells you may assume that all cells in the image should be marked with an
enclosing square. This is however, not always the case. If certain cells have been excluded from the cell