ChemoMetec NC-3000 - Manual (Page 5)

991-3003 Rev. 1.0 August 2013 5
Quick Guide
1. Install the NucleoView NC-3000™ software and the NucleoCounter® NC-3000™ instrument as
described in the documentation on the USB stick containing the NucleoView NC-3000™ software.
2. Start the NucleoView NC-3000™ software by double-clicking on the NucleoView NC-3000™ icon on
the desktop.
3. Wait while the NucleoCounter® NC-3000™ instrument initializes. The slide tray can be heard
aligning during this process. When the LED on the instrument turns green, the instrument is ready
to use.
4. Click the button (below the ‘F3’ button) and select the desired protocol.
5. Click the button to see the application note for the selected protocol.
Check that you have all the materials needed for this protocol (slides, reagents ...).
6. Mix your cell sample, add potential solution(s) and load a Cassette, A2 or A8 side.
7. Eject the sample tray and insert the slide or cassette.
8. Select the chambers to be analyzed by clicking in the checkboxes.
9. Optional: Write a descriptive text in the Sample ID field and enter a
user name in the Operator field.
10. Press the ‘Run’ button.
The analysis will now be started and the results of the analysis will be displayed a few moments
later.
Repeat steps 6 to 10 to perform the same protocol again on a new sample or repeat from step 4 to
start a new type of analysis.