Eppendorf Multiporator - Manual (Page 18)

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4.8 Cell fusion (optional)
4.8.1 Micro fusion chamber (Electrode gap width 0.2 mm)
With the help of the Micro fusion chamber, the parameters for the cell
alignment and for the cell fusion can be optimized under microscopic
control.
The Micro fusion chamber consists of a housing equipped with a
transparent reservoir, into which two electrodes with a gap of 200 µm are
embedded. The connection to the Multiporator takes place through a
coaxial cable (approx. 1 m) over a special insert (see Fig. 5).
Fig. 5: Schematic diagram of the insert and the Micro fusion chamber
Caution : The Micro fusion chamber must be handled with care.
Take care when filling with cell suspension. Do not touch or move the
electrodes with the pipette tip. This may cause damage!
During cell alignment/fusion, avoid direct contact with the
electrodes. Cell alignment/fusion may be performed only when
the micro fusion chamber is connected.
– Slide the Micro fusion chamber firmly into the Multiporator

.
– Place the Micro fusion chamber (which is filled with approx. 20–50 µl
cell suspension) onto the microscope.
Note : Pipetting the cell suspension carefully onto the area around the
electrodes can maximize the number of cells between the electrodes.
– The brackets can be used
to lock into place on the
microscope table. They
can also be used to
fasten a glass slide.
1 2 4
Insert of Micro fusion chamber
1 Contacts
2 Earthing springs
Micro fusion chamber
4 Coaxial connector
3 Cable
3 3
4 Device description
Multiporator_Text_en.fm Seite 60 Freitag, 9. Juni 2006 8:05 08