4 device description – Eppendorf Multiporator User Manual
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When screwing the core, make sure that no air bubbles form.
Fig. 10: Filling the Helix fusion chamber (schematic representation)
Leave the closed Helix fusion chamber upside-down on the coaxial
connector until the fusion process has been completed!
4.8.6 Fusion and cell suspension extraction
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Slide the fusion insert firmly into the device.
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Place the closed Helix fusion chamber onto the coaxial connector
and lock it into place by rotating it a quarter-turn.
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Carry out the fusion with the set parameters (see chap. 5.3).
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Loosen the Helix fusion chamber carefully by rotating it a quarter-turn
and then remove it.
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To open the chamber, place it in a vertical position (e.g. in the stand).
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Unscrew the core carefully from the beaker.
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Using a small quantity of the appropriate medium, rinse off any cell
suspension that may be on the core.
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The cell suspension from the beaker is mixed with the cell suspension
that has been rinsed off and is then treated further as required.
4 Device description
Multiporator_Text_en.fm Seite 64 Freitag, 9. Juni 2006 8:05 08