Eppendorf Multiporator - Manual (Page 21)

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The gap between the wound platinum electrodes is approx. 200 µm.
Fig. 9: Microscope view of the electrodes of the Helix fusion chamber
Caution : Handle the Helix fusion chamber with care!
Loose or damaged electrodes adversely affect operating procedures.
In such cases, the Helix fusion chamber must not be used!
4.8.5 Filling the Helix fusion chamber
The Helix fusion chamber is filled with 250 µl cell suspension. It is
essential to ensure that the liquid is pipetted into the deepest possible
position in the cup. The edge and the walls must not be wetted as this has
an adverse effect on filling the Helix fusion chamber. Air bubbles may form
which reduce the effectivity of the experiment.
The core is inserted into the upright cup in the stand and carefully
screwed into place. As a result of this screwing action, the cell suspension
in the ever-decreasing gap is forced upwards.
200 µm
4 Device description
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