Eppendorf Multiporator - Manual (Page 35)

77
5.3.2 Procedure for cell fusion
Step 1 – Entering / modifying parameters
– Press the SET
key to select the
parameters desired
(the parameter will
be underlined).
– Use the arrow
keys to modify the
parameter in the
direction required.
According to the
parameters selected
for U'
~
and U"
~
, fusion
can be carried out
without alignment or
in combination with
one or both
alignment functions
as required.
– Terminate the
entering procedure.
The parameters
are effective
immediately and
do not have to be
stored.
Example of a display of
electrofusion with activa-
ted alignments U'
~
/ U"
~
.
Example of the display
for carrying out elec-
trofusion with inactiva-
ted alignment U'
~
.
The relevant parameters
appear in the display in
larger letters and in bold
print.
U'
~
/ U"
~
:
Alternating voltage, in
V (volt).
Can be set to "0" and
in increments of 0.1 V
in the range 1.0–10 V.
t: Duration of alignment
0–95 s, in
s (seconds). Can be
set in increments of
5 seconds.
U : Voltage, in V (volt).
Can be set to "0" and
in increments of 1 V
between 5 V and
100 V, and in incre-
ments of 10 V be-
tween 100 V and
300 V.
t : Duration of pulse,
in µs (microseconds).
Can be set in
increments of 5 µs
between 0 and
15 µs–300 µs.
n: Number of pulses
0–99.
Can be set in one-
pulse increments.
12.AUG.00 13:42
U'
~
5V t 30s
U 30V t 15µs n2
U "
~
5V t 30s
12.AUG.00 13:42
U'
~
0V t 30s
U 30V t 15µs n2
U "
~
5V t 30s
5 Mode of operation
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