Eppendorf Multiporator - Manual (Page 24)

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4.9 Insert (electroporation / electrofusion)
for connecting external electrodes
Prior to using the insert for the connection of external
electrodes, the supplement sheet accompanying the insert
must be read thoroughly. The safety instructions found in the
supplement sheet must be observed.
The non-standard accessories (see "Ordering information") contain an
insert, to which it is possible to connect external electrodes. By changing
the position of a function switch (
P / F ) accordingly, the insert can be used
for electroporation (symbols ) or for cell fusion with external
electrodes (symbol ).
The electrodes are connected using two 4 mm contact-proof
laboratory plugs.
Fig. 11: Insert for the connection of external electrodes (schematic representation)
Attention! Characteristic of electrofusion of cells with
external electrodes:
During the electrofusion, a resistance of 50 Ohm must be
connected parallel to the electrodes with the help of plug in
connections.
1
1
2
2
1 Connecting socket, positive pole (red)
2 Connecting socket, ground (black)
Position of the function switch for electrofusion F
Position of the function switch for electroporation P
4 Device description
Multiporator_Text_en.fm Seite 66 Freitag, 9. Juni 2006 8:05 08