5 mode of operation – Eppendorf Multiporator User Manual

Page 33

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5.3 Mode for cell fusion (optional)

This mode is represented in the display by the symbol

Instructions for the usage of the Micro fusion chamber and the Helix
fusion chamber have been summarized in chapter 4.8 of the operating
instructions. A detailed description of the electrofusion of eukaryotic cells
can be found in the Basic Applications Manual for electrofusion, which is
included in the delivery package of the fusion module. This application
manual can also be downloaded from the Eppendorf Homepage
www.eppendorf.com.

5.3.1 Function: Cell alignment

The functions for alignment are indicated by the symbols U'

(before the

pulse, alignment) and U"

(after the pulse, post-alignment) in lines 2 and 4

of the display.

Step 1 – Entering / changing parameters

–

If necessary, switch
to the mode by

pressing the MODE
key.

–

Press the SET key
to select the
parameter desired
(the parameter will
be underlined).

–

Use the arrow keys
to modify the
parameter in the
direction required.
If a value in lines
U'

/ U"

U are

set to "0", the
function in question
is not carried out.
This enables U'

to be triggered

individually or
consecutively
without the fusion
pulse being
activated.

Example of the display for
cell alignment U'

with-

out activation of pulse U
and alignment U"

The relevant parameters
appear in larger letters
and in bold print.

/ U"

Alternating voltage, in
V (volts). Can be set
to "0" and in incre-
ments of 0.1 V be-
tween 1.0 V and 10 V.

t: Duration of alignment

0–95 s, in s (seconds).
Can be set in incre-
ments of 5 seconds.

U : Voltage,

(square-wave pulse)
unit V (volts).
Can be set to "0" and
in increments of 1 V
between 5 V and
100 V, and in
increments of 10 V
between 100 V and
300 V.

12.AUG.00

13:42

60s

15s

30s

5 Mode of operation

Multiporator_Text_en.fm Seite 75 Freitag, 9. Juni 2006 8:05 08

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