5 mode of operation – Eppendorf Multiporator User Manual
Page 36
78
Step 2 – Starting cell fusion
–
Trigger the cell
fusion process
by Pressing the
START key.
–
"Charge" appears
briefly in the fourth
line of the display.
Charging
commences.
–
During the charging
procedure,
alternating voltage
U'
~
is applied for the
selected duration.
The alternating
voltage is
represented by the
symbol
~
(sine-wave
oscillation).
–
Discharge occurs
after the pre-
counted alignment
time (0–95
seconds). This is
indicated by a flash
in the fourth line of
the display.
If the number n
which has been
entered is n >1,
a further discharge
occurs each time
after one second.
12.AUG.00
13:42
U'
~
5V
t
30s
U
30V
t
15µs
n2
Charge
12.AUG.00
13:42
0:23
U'
~
5V
t
30s
U
30V
t
15µs
n2
5 Mode of operation
Multiporator_Text_en.fm Seite 78 Freitag, 9. Juni 2006 8:05 08