Leica Biosystems LN22 - Manual (Page 27)

27 Leica LN22
4.8 Specimen holders
During operation metal surfaces and components inside the
cryochamber can reach temperatures from as low as -196°C up to
as hot as +110°C!
Touching these extremely cold or hot parts can cause severe in-
jury!
For that reason, for any type of manipulations inside the
cryochamber you may use only the special tools which come as
part of the standard delivery and the use of which is described in
detail in the present manual. Transfer of frozen specimens may also
only be done by means of the corresponding special tools.
Always attach the specimen holder before inserting the knife
holder!
1 Freezing station
2 Cryo specimen arm
3 Clamping screw of the cold bridge
4 Cold bridge
5 Specimen holder
4.8.1 Specimen holder for block
embeddings
• Put the specimen together with appropriate
embedding medium (e.g. 2.3 molar saccharose
solution) into the specimen holder.
4 3
1
5
2
Fig. 19
Fig. 20
OCT is very rarely suitable for these
low temperatures!
4. Startup