Leica Biosystems LN22 - Manual (Page 31)

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Inserting the knife holder
Always attach the specimen holder before inserting the knife
holder to avoid damaging the knife!
To insert the knife holder into the cryochamber, always use the ap-
propriate manipulator, which comes as part of standard delivery!
• Place knife holder (2) using manipulator (4) diagonally from above
onto knife holder base (9, Fig. 24). Always use the manipulator in or-
der to avoid injury.
• Set the desired clearance angle (see Fig. 24).
Adjusting the clearance angle
The clearance angle is adjusted with the no. 3 Allen key, through the
drillings (16, Fig. 24) in the clearance angle adjusting on the left of the
knife holder.
Limit stop = 2° effective clearance angle 1st scale mark: 5°
2nd scale mark: 10°
3rd scale mark: 15°
• Fasten knife holder (5, Fig. 24) in knife holder base (9, Fig. 24) with
screws (6, Fig. 24).
• Once the selected temperature has been reached, retighten the
screws (7, Fig. 24), on the knife holder (5, Fig. 24), and on the knife
holder base (10, Fig. 24) as well as the knife clamping screws
(6, Fig. 24).
4. Startup
• Move the specimen cylinder to the rear limit stop.
• Insert knife (1) in the center of knife holder (2); tighten clamping screws
(3). If the knife is moved to the left or right edge, tighten only the center
and left or right screw (place remaining screws and tighten slightly).
1
4
2
3
Fig. 25