1 starting a staining run, Operation – Leica Biosystems ST4020 User Manual

Page 32

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Instructions for Use V1.1, RevB – 10/2012

5.

Operation

When the RUN key is pressed, the flushing valve is actuated, the
display changes and the remaining processing time appears,
counting downwards:

When a staining run is started by pressing the RUN key, the
system receives the information that there are slides in the start
position that are to be stained.

5.5.1 Starting a staining run

It is, of course, the user's responsibility to verify that each of the setup parameters (station
time, number of steps, number of dips, etc.) is set to an acceptable value before slides are
placed in the starting position and RUN is pressed.

Slides should not be loaded while the user is verifying setup parameters, because changing
the number of steps ("transfer mechanism calibration") will cause the transfer mechanism to
advance to the next position!
All parameters should be verified BEFORE loading the first set of slides.

A staining run cannot be started unless the stainer is in standby-mode.

In this mode, the system displays the process time and instructions to press the RUN key to
start:

Note that the first station's process time begins as
soon as the RUN key is pressed. This means that the
slide carriers must be loaded in the start position
immediately before the RUN key is pressed.

The stainer will continue running until these slide carriers are
transferred from station 14 to the exit tank.

The current operation appears successively in the display.

When the last slide carrier has been transferred to the exit
tank, the system stops the process and returns to IDLE mode,
unless more slides are inserted in the correct manner.

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