Metrohm 833 IC Liquid Handling Unit - Manual (Page 36)

4 833 IC Liquid Handling Pump Unit
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
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1 Connect column to injector
• Remove end caps from column 53.
• without column heating:
Screw inlet end of separating column 53 (note flow direction)
to column connection capillary 50 mounted on the injector.
• with column heating:
Prepare column heating according to Section 2.9.2 of the
819/820 Instructions for Use and screw column connection
capillary 50 (see Fig. 20, 819/820 Instructions for Use) with a
compression fitting to injection valve 51.
• With precolumn:
Install precolumn according to the supplied leaflet between
inlet of the separating column and the injection valve.
2 Rinse column
• Place a beaker beneath the column outlet.
• Start 818 IC Pump in «IC Net» with suitable flow (see leaflet of
the column) and rinse column for ca. 10 min with eluent.
• Stop 818 IC Pump.
3 Connect column to suppressor module (Suppressor
connection 1)
• Cut inlet capillary 64 (marked with "Eluent") of suppressor
module 70 to the required length. Use the 6.2126.080
Capillary cutter available as an option.
• without column heating
Screw inlet capillary 64 on to the outlet end of separating
column 53 using a 6.2744.010 compression fitting.
• with column heating
Connect capillary 52 at the outlet of the separating column
(see Fig. 20, 819/820 Instructions for Use) to the inlet
capillary 64 using the 6.2744.040 Coupling.
4 Fix column
• without column heating
Insert one or two column holders 59 (6.2027.030, 6.2027.040
or 6.2027.050) in the mounting rails 58 and fasten separating
column in the column holder.
• with column heating
Insert column heating according to Fig. 10 (819/820
Instructions for Use) into the Separation Center.