Metrohm 833 IC Liquid Handling Unit - Manual (Page 74)

7 833 IC Liquid Handling Dialysis Unit
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
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7.7 Dialysis procedure
For determinations of samples by using dialysis it is advisable to
proceed in the following sequence:
1 Prepare acceptor solution
• To avoid interference from air bubbles in the acceptor
channel the ultrapure water used for the acceptor solution
should always be degassed for at least 10 min by vacuum,
N
2
or He.
2 Prepare sample
• In order to avoid blocking the sample channel, samples
containing a large amount of suspended or solid particles
should always be centrifuged using a benchtop centrifuge
at 10'000 min -1 for 5 min.
3 Start up IC system
• Switch on all instruments.
• Start the IC Net software and load/create the required system
(see «IC Net» Instructions for Use).
• Condition the IC column (see 819/820 Instructions for Use).
4 Start up dialysis system
• Immerse both pieces of aspiration tubing 16 in the acceptor
solution.
• Start the peristaltic pumps in «IC Net» by setting the remote
line 1 (833 Pump Unit) and remote line 2 (833 Dialysis Unit) in
the window of the 819 Detector.
• Rinse the dialysis system with acceptor solution for approx.
10 min and then switch off the pump again.
5 Calibration
• Immerse sample aspiration tubing 16 in the standard
solution.
• Start the determination in IC Net and wait until the standard
chromatogram has been evaluated and the next standard
can be measured.
6 Sample determination
• Immerse sample aspiration tubing 16 in the sample solution.
• Start the determination in IC Net and wait until the
chromatogram has been evaluated.