Bio-Rad Experion DNA Analysis Kits - Manual (Page 13)

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When placing a chip on the priming station, match the alignment arrow on the chip with the alignment
arrow on the chip platform (Figure 2.2). Alignment posts on the underside of the chip (next to the glass)
match holes in the chip platform to ensure correct positioning of the chips in the priming station.
The priming station uses time and pressure settings optimized for each different chip type (Figure 2.1).
Use the alphanumeric priming codes on the chip (Figure 2.2).
Fig . 2 .2 . Experion priming station
alignment arrows (left) and chip priming
code (right) .
Alignment arrows
Alphanumeric priming code
2.2.2 Vortex Station
The vortex station is used only for RNA and DNA applications. In these applications, sample and loading
buffer are loaded directly into the wells of the chips to minimize sample volume requirements. The
vortex cycle mixes the samples and buffers thoroughly before the chip is placed into the electrophoresis
station.
Connect one end of the power cable to the vortex station and the other end to a grounded AC outlet.
Press the green Power button on the front of the vortex station to power it on.
To place a loaded chip onto the vortex station, slide the chip under the prongs of the vortex adapter,
ensuring the chip is inserted all the way until it is seated inside the chip indentation (Figure 2.3). The chip
fits securely in the adapter.
Fig . 2 .3 . Experion vortex station . Left, inserting the chip. Right, chip on the vortex adapter.
To start the vortex step, press the Mix button. After 1 min, the vortex station stops and three beeps
indicate the end of the cycle. Remove the chip by depressing the tab on the adaptor area at the bottom
of the chip and sliding the chip out (Figure 2.4).
Experion Automated Electrophoresis System