Bio-Rad Experion DNA Analysis Kits - Manual (Page 25)

Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com 21
4.2 Priming Station
Error Probable Cause Recommended Action
LCD screen is blank There is no power to the priming station Check the power cord connections and for AC power to the
outlet
Check Seal message The priming seal is worn, torn, or clogged Replace the priming seal (see Section 3.2, Priming Station)
The chip is not positioned correctly in the
priming station Align the arrow on the chip with the arrow on the platform
The gel-stain solution (GS) was added to the
wrong well for priming The priming well is highlighted on the chip. Refer to the
assay manual for information on how to prime the chip
Nothing happens when the
Start button is pushed The gel-stain solution (GS) was added to the
wrong well for priming The priming well is highlighted on the chip. Refer to the
assay manual for information on how to prime the chip
Timer fails to count down
to zero after pushing the
Start button The priming seal is worn, torn, or clogged Replace the priming seal (see Section 3.2, Priming Station)
Service message and
wrench icon appear in
the display Contact your local Bio-Rad office
After priming, a bubble
is present in the
microchannels Poor application of gel-stain solution (GS);
bubbles that are forced into the microchannels
take the shape of the microchannel and are
elongated, not round Prime a new chip
After priming, channels
are not filled with gel-
stain solution (GS) Inappropriate priming pressure and time
settings were used Use the pressure and time settings indicated on the chip for
the selected assay
GS was added to the wrong well for priming The priming well is highlighted on the chip. Refer to the
assay manual for information on how to prime the chip
The priming seal is worn, torn, or clogged Replace the priming seal (see Section 3.2, Priming Station)
Particulates in GS are clogging the
microchannels Prepare new GS or refilter an older aliquot
After changing the priming seal, or if the display is not working properly, reset the priming station by
unplugging it for a few sec and then reconnecting the power cord.
Experion Automated Electrophoresis System