5 diagnostic tests of the electrophoresis station, 1 system diagnostic tests, 2 current test – Bio-Rad Experion DNA Analysis Kits User Manual

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Technical

Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com

4.5 Diagnostic Tests of the Electrophoresis Station

Experion software features a set of programmed diagnostic tests used to check various aspects of
electrophoresis station performance. The Experion validation kit (catalog #700-7051) or Security Edition
software (catalog #700-7052) are required to execute some of these tests. If the electrophoresis station
fails, repeat it for confirmation. If it fails twice, contact Bio-Rad Technical Support for assistance.

These tests are also executed by the IQ/OQ protocols as part of the Validation menu, which allows
you to print a hard copy of the pass/fail report.

4.5.1 System Diagnostic Tests
Perform these tests to perform functional analyses of various aspects of the system.

1. Under Tools > Diagnose Instrument, click Systems and select all of the following the diagnostic

tests:

n

Communications — reports the rate of data transfer (bytes/sec) between the

electrophoresis station and the application

n

Electronics — performs all startup electronic board tests for the electrophoresis station

n

Fan — verifies the fan is running

n

Heater Plate — verifies that the heater achieves a set temperature within an acceptable

time window (not before 30 sec and not after 5 min of the start of the command)

n

Lid Sensor — verifies the operation of the lid sensor

2. Click Start. Results appear in the Test Results field as pass or fail. If a test fails, record the

explanation and contact Technical Support.

4.5.2 Current Test
Perform this test to determine whether current is leaking between the electrode pins. This test requires
use of the leakage current chip included in the Experion validation kit.

1. Under Tools > Diagnose Instrument, click Current. The Instrument Diagnostics window appears.

2. Follow the instructions in the Instructions field. Check the instruction after you complete the

command. When all instructions are checked, the Start button is enabled.

3. Click Start. A 10 min countdown timer appears. Results appear in the Results field.

Experion Automated Electrophoresis System

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