Bio-Rad Experion DNA Analysis Kits - Manual (Page 6)

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1.1 System Overview
The Experion automated electrophoresis system (Figure 1.1) employs LabChip microfluidic technology to
automate protein and nucleic acid electrophoresis. It integrates separation, detection, and data analysis
within a single platform. Using much smaller sample and reagent quantities than standard analysis
methods, the Experion system accomplishes analysis in a single 30–40 minute, automated step.
The Experion system is used for the following applications:
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Protein analysis — quantitation and size determination (sizing) of protein samples useful for
quality control, protein purity and stability analysis, protocol optimization, and evaluation of
recombinant protein expression
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RNA analysis — evaluation of RNA integrity and concentration. The system automatically
generates the RNA quality indicator (RQI), a quantitative integrity assessment, in addition to
visual electropherogram data, ribosomal RNA ratios, and concentration data. This makes
Experion RNA asays useful upstream of applications such as real-time quantitative PCR
(qPCR), microarray analysis, sequencing, and reverse-transcription qPCR (RT-qPCR)
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DNA analysis — quantitation and sizing of restriction digests, amplified DNA,
microsatellites, and amplified fragment length polymorphisms (AFLPs)
For details about how the Experion system performs these analyses, refer to the instruction manuals for
each analysis kit.
Fig . 1 .1 . The Experion system . The system includes the following components: 1) automated electrophoresis
station, 2) priming station, 3) vortex station used for nucleic acid analysis only, 4) system operation and data analysis
tools (software), and 5) analysis kits, which include the (a) chips and (b) reagents for protein (Pro260 kit), standard-
sensitivity RNA (StdSens kit), high-sensitivity RNA (HighSens kit), and DNA (DNA 1K and 12K kits) analyses.
Experion Automated Electrophoresis System
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