Bio-Rad Experion DNA Analysis Kits - Manual (Page 9)

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Experion RNA StdSens and RNA HighSens analysis kits — offer rapid single-step
assessments of RNA quality and quantity and the ability to analyze 1–12 samples in
approximately 30 min. Sample concentrations in nanogram (StdSens) or picogram
(HighSens) amounts can be analyzed
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Experion DNA 1K and DNA 12K analysis kits — used for single-step sizing and quantitation
of DNA fragments in up to 11 samples
Table 1.1 Experion analysis kits.
Pro260 RNA HighSens RNA StdSens DNA 1K DNA 12K
# Samples 1–10 1–11 1–12 1–11 1–11
Sample volume 4 µl 1 µl 1 µl 1 µl 1 µl
Concentration range 5–2,000 ng/µl 100–5,000 pg/µl 5–500 ng/µl 0.1–50 ng/µl 0.1–50 ng/µl
Separation range 10–260 kD — — 15–1,500 bp 50–17,000 bp
Sensitivity 2.5 ng/µl 100 pg 5 ng 0.1 ng 0.1 ng
(carbonic anhydrase
in 1x PBS)
1.2.6 Experion Software
Experion software includes the controls required to operate the electrophoresis station and the data
analysis tools needed to assess the results. During a run, the software provides the ability to monitor run
progress or review previous sample results. For more information about the use of Experion software,
refer to the Experion analysis kit manuals.
Two optional software kits assess system performance and aid in troubleshooting:
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The Experion validation kit (catalog #700-7051) includes automated instrument qualification
(IQ) and operational qualification (OQ) protocols that test the critical functions of the system
and verify and validate specific system functionality; it is useful for troubleshooting
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Experion Security Edition software (catalog #700-7052) includes the IQ/OQ protocols of the
validation kit in addition to tools for compliance with U.S. FDA 21CFR Part 11 regulations
1.3 Experion Workflow
Sample analysis involves the following steps:
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Preparing the chip (priming, loading, and vortexing) — Priming fills the wells and
microchannels of the microfluidic chip with the gel-stain solution, which contains both the
sieving matrix and fluorescent dye. Samples prepared in Experion sample buffer are then
loaded in the sample wells. The chip is vortexed in RNA and DNA analyses
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Running the chip — The chip is inserted into the electrophoresis station, and as the
electrophoresis station lid is closed, electrodes come in contact with the solution in the
wells. The voltage applied to the sample wells causes the charged molecules to migrate
into the separation channel. In the separation channel, the gel-stain solution causes the
molecules to move at different rates, depending on their size. During separation, the
fluorescent dye, which is specific to the analysis being performed, either associates with
the lithium dodecyl sulfate (LDS) coating the proteins (protein analyses) or intercalates
between the bases of RNA (RNA analyses) or DNA (DNA analyses)
Experion Automated Electrophoresis System