Percent max binding assay tutorial, 1 defining the template, 1defining the template – Bio-Rad Microplate Manager Software User Manual

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Appendix

92

Percent Max Binding Assay Tutorial

1 Defining the Template
2 Setting up the Equations
3 Selecting Curve Fit and Customizing Curve Fit Plot
4 Entering Experimental Info
5 Setting up the Report
An example of how to set up an ELISA Assay including a standard curve and
equations to transform the absorbance data into percentage values of the maximum
bound well is given here.

Open sample data file (Percent Max Binding.mpl) with its protocol and template

C:\Program Files\Bio-Rad\Microplate Manager 6\Sample Data

1

Defining the Template

1. Click on the Template icon in the Main Toolbar

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