Bio-Rad Microplate Manager Software - Manual (Page 33)

Setting up a Protocol
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Setting Up A Template
The template defines the identifier locations for your plate data. A template must
always be present in order to see any Result Data or Report window.
The template controls allow you to edit your template, as well as to enter equations
and dilutions. The controls are available as menu items, or as icons under the
Template Window toolbar.
Click on the Template icon in the Main toolbar or choose Template under the
Window menu to open a template.
Blanks, standards or sample identifiers are entered in the template for each well.
Samples Begin Sample IDs with an alphanumeric letter, for example,
POS or NEG (positive or negative control), or MAX (well
with a maximum sample value). Any long sample ID or
barcode can be entered. Sample wells are shown in blue.
Standards Enter standard concentration values as positive numbers.
Standard wells are shown in red.
Blanks Enter the number sign, # (shift 3) for each blank well. Blank
wells are green with #. The average of all blank wells with
“#” will automatically be subtracted from the raw data.
A second blank can be defined as BLK, if desired, and
equations can be set up to do the subtraction. (see Percent
Control Assay Tutorial on page 82)
Unused
Wells Leave unused wells empty.
To fill in a template, click on a well and enter identifier for that well. To move
from well to well, use the arrow keys or the tab key.
To use the Fill features, highlight a section of the template and select Fill Down,
Fill Right, or Fill Series.