Bio-Rad Microplate Manager Software - Manual (Page 61)

Running Kinetic Assays
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3. Enter the interval between the start of two consecutive reads in the Interval
column.
The interval time is the time necessary for one read plus mix time specified.
4. Enter mix time, if desired.
In the above kinetic read example, the reader will do 40 readings with 30 second
intervals and no mix.
5. After the parameters are set, click the Read Plate icon to start the readings.
Analyzing Kinetics Data
When the raw kinetic data have been collected, a Kinetic Plots screen will appear.
This shows response versus time plots for the reactions in all the wells.
The raw data are shown in the initial displays as the total run time, beginning from
time 0 to the end of the run time.