Bio-Rad Microplate Manager Software - Manual (Page 43)

Analyzing Data Using a Standard Curve
35
Semi-log Fit
When Semi-Log is selected in the Curve Fit dialog box, a plot of the response (y
axis) vs. the logarithm of the concentration (x-axis) is obtained as shown below:
For the semi-log fit, the equation is:
y = Slope*Log
10
(conc)+ Intercept
The calculated intercept, slope, the correlation coefficient (r) and chi 2 values of the
line are shown below the plot.
The semi-log standard curve fitting option should be used when a linear plot of the
data exhibits a strong curvature up or down.