2 setting up the equations, 2setting up the equations – Bio-Rad Microplate Manager Software User Manual
Page 101
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Appendix
93
• Blanks are defined as #. The average of these # wells will be
subtracted from all the wells.
• NSB are non-specific binding wells.
• MAX is for the maximum binding well (i.e. the Bo well).
• S01 to S21 are all of the unknown samples to be analyzed.
2
Setting up the Equations
1.
Click Equation Editor icon in the Template Toolbar.
2.
Choose % MAX from Name popup to view equations to be applied
sequentially to data
•
@ - # — Subtracts mean of blank (#) wells
•
@ - NSB — Subtracts mean of non specific binding (NSB) wells
•
@ / MAX — Divides by mean of maximum binding (MAX) wells
•
@ * 100 — Multiplies by 100 to convert to % bound
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