Dilution data assay tutorial, 1 defining the template, 1defining the template – Bio-Rad Microplate Manager Software User Manual

Page 76

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Appendix

68

Dilution Data Assay Tutorial

1.

Defining the Template

2.

Setting up the Dilutions

3.

Selecting Curve Fit and Customizing Curve Fit Plot

4.

Entering Experimental Info

5.

Setting up the Report

An example of how to set up an ELISA Assay including a dilutions and a standard
curve is given here. The Report is customized to show interpolated concentration,
dilution factor, conc X dil factor, and mean concentration for the samples.

Open sample data file (Dilution Data.mpl) with its protocol and template

C:\Program Files\Bio-Rad\Microplate Manager 6\Sample

Data

1

Defining the Template

1. Click on the Template icon in the Main Toolbar

2. Type 200 in well A1 and press Enter

3. Highlight area well A1-G2. (Click & hold mouse on well A1, drag

mouse to well G2, and release.)

4. Click on the Fill Series icon in the Template Toolbar

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